I would first test between R and C wires. Do you get 24VAC roughly?
If not, there may be an issue with the main HVAC terminals. If there is 24VAC, that may mean the Y wire is disconnected, or an issue at the AC.
I would also try deleting and resetting a thermostat, then reinstalling to see if the setup process picks up the right wires and if this issue still occurs.
Did you recently see any Safety Delay or Isolation Relay Required messages?
On the thermostat:
- Press the Menu button.
- Select SETTINGS.
- Select INSTALLER.
- Select CONTINUE.
- Select RESET.
- Select YES.